Label-free immunosensing of telomerase using bio-conjugation of biotinylated antibody to poly(chitosan) gold nanoparticles: supplementary figures

Aim: This study aimed to establish a label-free electrochemical biosensor for telomerase detection

in human biofluid. Method: Synthesized green nanocomposite (poly[chitosan] decorated by gold

nanoparticles) was used for the efficient immobilization of biotinylated antibody of telomerase

and immunocomplex of antigen-antibody. Poly(chitosan) was decorated by gold nanoparticles on

the surface of a glassy carbon electrode using an electrochemical coating technique. Results: The

constructed immunosensor exhibited wide dynamic range (0.078–160 IU/ml-1) with a low limit of

quantification of 0.078 IU/ml-1, which present a unique manner for telomerase assays in early prognosis

for cancers. Conclusion: This study encourages scientists and scholars to design and develop new biosensor

platforms for point-of-care diagnostics for telomerase management, an interesting reference for future

research.