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Label-free immunosensing of telomerase using bio-conjugation of biotinylated antibody to poly(chitosan) gold nanoparticles: supplementary figures

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posted on 2023-05-12, 07:46 authored by Ahmad Mobed, Fereshteh Kohansal, Sanam Dolati, Mohammad Hasanzadeh

Aim: This study aimed to establish a label-free electrochemical biosensor for telomerase detection

in human biofluid. Method: Synthesized green nanocomposite (poly[chitosan] decorated by gold

nanoparticles) was used for the efficient immobilization of biotinylated antibody of telomerase

and immunocomplex of antigen-antibody. Poly(chitosan) was decorated by gold nanoparticles on

the surface of a glassy carbon electrode using an electrochemical coating technique. Results: The

constructed immunosensor exhibited wide dynamic range (0.078–160 IU/ml-1) with a low limit of

quantification of 0.078 IU/ml-1, which present a unique manner for telomerase assays in early prognosis

for cancers. Conclusion: This study encourages scientists and scholars to design and develop new biosensor

platforms for point-of-care diagnostics for telomerase management, an interesting reference for future

research.

Funding

This study was financially supported by the Aging Research Institute, Tabriz University of Medical Sciences, Tabriz, Iran (grant no. 66119), and Pharmaceutical Analysis Research Center, Tabriz University of Medical Sciences, Iran.

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