Knockdown of lncRNA AC245100.4 inhibits the tumorigenesis of prostate cancer cells via STAT3/NR4A3 axis
Fig. S1. The relative mRNA expression of several genes related to tumor progression was detected via the knockdown of AC245100.4. Fig. S1. (A) The up/down regulation genes were sequenced according to the rank of differentially expressed in the RNA-seq analysis. (B) The relative mRNA expression was detected by qRT-PCR in PC3 and DU145 cells with AC245100.4 knocked down. vs. Nc. * P<0.05, ** P<0.01, *** P<0.001. Fig. S2. Overexpression efficiency was detected in PC3 and DU145 cell lines. Fig. S2. (A&B) Overexpression efficiency was detected by qRT-PCR and western blot in PC3 and DU145 cell lines. * P<0.05, ** P<0.01. |
Fig. S3. The effect of NR4A3 on the proliferation of prostate cancer cells. (A&B) The proliferation of PC3 and DU145 cells was determined by CCK-8 and colony formation assays following overexpression of NR4A3. vs. Nc. ns, not significant. |