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Knockdown of lncRNA AC245100.4 inhibits the tumorigenesis of prostate cancer cells via STAT3/NR4A3 axis

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posted on 18.10.2021, 08:24 by Chi Liu, Ping Lin, Jiabin Zhao, Hui Xie, Rou Li, Xu Yang, Nan Wang, Huizhen Jia, Shan Jiang, Ke Zhang, Xiaoguang Yu

Fig. S1. The relative mRNA expression of several genes related to tumor progression was detected via the knockdown of AC245100.4.

Fig. S1. (A) The up/down regulation genes were sequenced according to the rank of differentially expressed in the RNA-seq analysis. (B) The relative mRNA expression was detected by qRT-PCR in PC3 and DU145 cells with AC245100.4 knocked down. vs. Nc. * P<0.05, ** P<0.01, *** P<0.001.

Fig. S2. Overexpression efficiency was detected in PC3 and DU145 cell lines.

Fig. S2. (A&B) Overexpression efficiency was detected by qRT-PCR and western blot in PC3 and DU145 cell lines. * P<0.05, ** P<0.01.

Fig. S3. The effect of NR4A3 on the proliferation of prostate cancer cells.

Fig. S3. The effect of NR4A3 on the proliferation of prostate cancer cells. (A&B) The proliferation of PC3 and DU145 cells was determined by CCK-8 and colony formation assays following overexpression of NR4A3. vs. Nc. ns, not significant.

Funding

Fundamental Research Funds for the Provincial Universities (No. 2018-KYYWF-0432)

Postdoctoral Scientific Research Developmental Fund of China (No. 212538)

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