Influence of C107R mutation from hepatitis B virus genotype H on in vitro hepatitis B surface antigen detection and IFN-β-1a treatment: Supplementary figures
Aim: Assess the in vitro effect of hepatitis B virus (HBV) genotype H (HBV/H) with the small surface HBV
protein (HBs) C107R mutation on hepatitis B surface antigen (HBsAg) detection, TGFB1, CAT and IFNB1A
expression, and the response to IFN-β-1a treatment. Methods: HBV/H wild-type and HBs C107R variant
replicons were constructed and transfected into hepatic stellate cells and/or Huh7 that were later
treated with IFN-β-1a. HBsAg, HBV-DNA, pgRNA, TGFB1, CAT and IFNB1A expression was analyzed.
3D HBs structure from wild-type and C107R were foreseen by AlphaFold protein predictor, and IFN-β-
1a antiviral effect was evaluated. Results: C107R mutation did not impact viral replication, but HBsAg
serologic detection was affected.Wild-type and C107R similarly modified gene expression and responded
to IFN-β-1a. Conclusion: C107R disrupts the Cys107/Cys138 disulfide bond and impairs HBsAg detection.
Independently of the mutation, there were changes in TGFB1, CAT and IFNB1A expression, and a medium
response to IFN-β-1a treatment compared with genotype A and C.