Future Science Group
Browse
Supplemental figures.pptx (6.1 MB)

Figures - Efficient Multiplexing and Variant Discrimination in RT-LAMP with Sequence-specific Hybridization Probes

Download (6.1 MB)
figure
posted on 2022-11-02, 10:24 authored by Yinhuan Zhang, Nathan A. Tanner

      

Figure S1. Detection of   sequence variation S413R and LPP deletion. RT-LAMP were performed with   relevant primer set in the presence of WT (HEX) or the variant (FAM) probes.   For each panel, the amplification signal is shown on the left and melting   curve analysis on the right. (A) WT S413 probe (HEX) detected signal from RNA   Control 2 but not in RNA Control 50. (B) S413R probe (FAM) detected signal   from RNA Controls 50 but not in RNA control 2. (C) WT LPP probe (HEX)   detected signal from RNA Control 2 but not in RNA control 50. (D) LPPdel   probe (FAM) detected signal from RNA Controls 50 but not in RNA control 2. 

Figure S2. Detection of   sequence variation RGtoKR and HV deletion. RT-LAMP were performed with   relevant primer set in the presence of WT (HEX) or the variant (FAM or Cy5)   probes. For each panel, the amplification signal is shown on the left and   melting curve analysis on the right. (A) WT RG probe (HEX) detected signal   from RNA Control 2 but not in RNA control 50. (B) RGtoKR probe (FAM) detected   signal from RNA Controls 50 but not in RNA control 2. (C) WT HV probe (HEX)   detected signal from RNA Control 2 but not in RNA controls 14 or 48. (D) B117   HVdel probe (FAM) detected signal from RNA Controls 14 but not in RNA   controls 2 or 48. (E) BA1 HVdel probe (FAM) detected signal from RNA Controls   48, weak signal in control 14 and no signal in RNA controls 2. 

History

Usage metrics

    BioTechniques

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC