Evaluation of cellular stress responses in magnetomotive ultrasound Supplementary figure

Early and accurate diagnoses are important for successful cancer treatment. Lymph node involvement

is often critical, and magnetomotive ultrasound (MMUS) has been proposed for its detection and

characterization. MMUS relies on a magnetic contrast agent, for example, iron oxide nanoparticles,

delivered to the tissue of interest, magnetically set in motion and detected using ultrasound.

The magneto–mechanical interaction has not previously been evaluated on a cellular level. Here

we demonstrate uptake and dose-dependent retention of magnetic nanoparticles in two human

adenocarcinoma cell lines, with <10% cytotoxicity which did not increase following magnetic excitation.

Further, the oxidative stress levels were not affected by magnetic particles or force. Thus, we found no

evidence of adverse effects from the magneto–mechanical interactions under these conditions.