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Astragalus and human MSCs promote wound healing by mediating immunomodulatory effects through paracrine signaling. Supplementary figures

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posted on 07.03.2022, 09:27 authored by Qiang Tong, Patrick McHugh, Jiaqi Wang, Dandan Zhang, Wenchun Qu, Ying Zhu

Figure S1. Identification of Mesenchymal Stem Cells (MSCs)

MSCs were identified by marking positive with CD73 and CD90, and negative with CD45 and HLA-DR.

Figure S2. Quantitative test for Ethanolic extract of Astragalus (EEA)

EEA were reacted with 5% sodium nitrite and detected with an ultraviolet spectrophotometer. The absorbance was observed at 256nm, and a standard curve was drawn by the concentration of the standard product of EEA. X presents wavelength, Y standards absorbance. A: The spectrum drawn by the UV spectrophotometer. NC: normal control; Standard 1-4: the standard product of EEA; Raw: raw material; Sample1-4:EEA. B: The standard curve drawn with standard products of EEA.

Figure S3. Microvessel density test

A: CD31 antibody was used to stain the vascular cells. Immunofluorescence detection for CD31showed vascular cells increased after treatment of the mixture. Blue: DAPI; Green, CD31. B: Statistical analysis was performed for CD31+ cells and analyzed with ImagePro to evaluate the differences in mean vessel density.

Funding

The role of Nogo-B in regulating alveolar epithelial to mesenchymal transition in pulmonary fibrosis and its mechanism

National Natural Science Foundation of China

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Mechanism of A20 Regulating NF-κB Effect on Ligament ossification in Mouse Model of Ankylosing Spondylitis

National Natural Science Foundation of China

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2021ZD005

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