supplementary data High expression of DNAJA1 (HDJ2) predicts unfavorable survival outcomes in breast cancer.pdf

Supplemental Table S1. Sample information sheet of the commercial tissue microarray (TMA) used in the study TMA (BC081116b): Breast carcinoma tissue microarray with adjacent breast tissue, containing 100 cases of breast invasive ductal carcinoma and 10 cases of adjacent breast tissue, single core per case (Biomax Inc. Rockville, MD, USA)

Supplemental Table S2. Sample information sheet of the commercial tissue microarray (TMA) used in the study TMA (BR486): Breast invasive ductal carcinoma tissue microarray with matched adjacent normal mbreast tissue, containing 16 cases of breast invasive ductal carcinoma with matched adjacent normal breast tissue, triplicate cores per case (Biomax Inc. Rockville, MD, USA)

Supplemental Table S3. Primers used for mutation analysis

Supplemental Table S4. GEO and Array Express datasets used for survival (OS, RFS and DMFS) analyses by KM-plotter

Supplemental Table S5. Associations between DNAJA1 protein expression and clinical characteristics of invasive breast carcinoma samples

Supplemental Figure S1. Representative photographs from DNAJA1 immunohistochemistry study performed with commercial tissue microarrays.

Supplemental Figure S2

Kaplan-Meier plots of OS (a), RFS (b) and DMFS (c) in breast cancer subtypes according to DNAJA1 mRNA expression.

Supplemental Figure S3

(a) Heterozygous SNP (rs2297218) in the promoter region of DNAJA1 in SK-BR-3 cell line. “SK-BR-3 (F)”: Sequencing with forward primer; “SK-BR-3 (R)”: Sequencing with reverse primer; “Ref. Seq”: DNAJA1 genomic sequence (NCBI Gene ID: 3301) (b) The position of the SNP relative to the promotor

motifs and location of the primers are depicted. Promotor motifs are estimated by EPD “Search Motif Tool” from -2000 bp to 100 bp relative to TSS (P value = 0.001) (https://epd.epfl.ch//index.php).

Supplemental Figure S4

Bisulfite-modified sequences of the DNAJA1 gene analysed by COBRA assay.

Supplemental Figure S5

Kaplan-Meier plot of Overall Survival in breast cancer according to DNAJA1 protein expression (n=65) (Uniprot ID: P31689) (Györffy et al., 2010; Tang et al., 2018)