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Zohar Cretnik et al. R1 Supplementary material.docx (164.16 kB)
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fmb-2021-0288 supplementary data

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posted on 2022-07-26, 09:01 authored by Tjaˇsa ˇZ Cˇ retnik, Matjazˇ Retelj, Sandra Janezˇ ic, Aleksander Mahnic, Tine Tesovnik, Robert ˇSket, Barbara J Bizjan, Samo Jeverica, Mateja Ravnik, Mitja Rak, Mateja Borinc, Maja Rupnik, Tadej Battelino, Marko Pokorn, Jernej Kova

Aim: To evaluate the accuracy of two PCR-based techniques for detecting SARS-CoV-2 variant Alpha

(B.1.1.7). Materials & methods: A multicenter prospective cohort with 1137 positive specimens from

Slovenia was studied. A mutation-based assay (rTEST-COVID-19 qPCR B.1.1.7 assay) and amplification curve

pattern analysis of the Allplex SARS-CoV-2 assay were compared with whole-genome sequencing. Results:

SARS-CoV-2 variant Alpha was detected in 155 samples (13.6%). Sensitivity and specificity were 98.1 and

98.0%, respectively, for the rTEST-COVID-19 qPCR B.1.1.7 assay and 97.4 and 97.5%, respectively, for

amplification curve pattern analysis. Conclusion: The good analytical performance of both methods was

confirmed for the preliminary identification of SARS-CoV-2 variant Alpha. This cost-effective principle for

screening SARS-CoV-2 populations is also applicable to other emerging variants and may help to conserve

some whole-genome sequencing resources.


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