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Towards epigenetic regulation of triple-negative breast cancer via ligand-mediated nanoparticles - supplementary material

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posted on 2023-05-11, 10:10 authored by Guilherme F Picheth, Gabriela C Cardoso, Michelle B Collini, Joao OC Filizzola, Leonardo B Colauto, Cleverton L Pirich, Giovana G Nunes, Thiago Alessandre da Silva, Flavia LD Pontes, Ingrid F Zattoni, Edneia ASR Cavalieri, Giseli Klassen

Aims: Develop and analyze triple-negative breast cancer targeted nanoparticles loaded with the

demethylating agent decitabine. Materials & methods: The polymers were synthesized by ring-opening

polymerization of D,l-lactide and formulated into nanoparticles via emulsion-evaporation method. The

nanoparticles were characterized by physicochemical analysis as well as in vitro using breast cancer

cell lineages. Results & conclusion: The targeted nanoparticles exhibited a hydrodynamic diameter of

75 ± 12 nm, zeta potential -6.3 ± 0.2 mV and spherical morphology, and displayed greater in vitro

accumulation into MDA-MB-231 (triple-negative breast cancer cell-line) compared with MCF7 and HB4A

cell lineages as verified by fluorescence confocal microscopy and significant demethylating effects via

ADAM33 screening by PCR.

Funding

Fundação de Amparo à Pesquisa do Estado de Minas Gerais, (Grant / Award Number: )

Conselho Nacional de Desenvolvimento Científico e Tecnológico, (Grant / Award Number: )

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, (Grant / Award Number: )

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Keywords

EGF epigenetic nanoparticles triple-negative breast cancer

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CC BY 4.0

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