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Synergistic effect of hydralazine associated with triazoles on Candida spp. in planktonic cells - Supplementary material Table

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posted on 2023-08-04, 15:06 authored by Francisca BSA do Nascimento, L´ıvia GA Valente S ´, Jo˜ao B de Andrade Neto, Let´ıcia S Sampaio, Helaine A Queiroz, Lisandra J Silva, Vito´ ria PF Cabral, Daniel S Rodrigues, Sidsayde C Pereira, Bruno C Cavalcanti, Jacilene Silva, Emmanuel S Marinho, Helcio S Santos, Manoel O Moraes, He´ lio VN Ju´ nior, Cec´ılia R Silva

Objective: To evaluate the antifungal activity of hydralazine hydrochloride alone and in synergy with azoles against Candida spp. and the action mechanism. Methods: We used broth microdilution assays to determine the MIC, checkerboard assays to investigate synergism, and flow cytometry and molecular docking tests to ascertain action mechanism. Results: Hydralazine alone had antifungal activity in the range of 16–128 μg/ml and synergistic effect with itraconazole versus 100% of the fungal isolates, while there was synergy with fluconazole against 11.11% of the isolates. There was molecular interaction with the receptors exo-B(1,3)-glucanase and CYP51, causing reduced cell viability and DNA damage. Conclusion: Hydralazine is synergistic with itraconazole and triggers cell death of Candida spp. at low concentrations, demonstrating antifungal potential.

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