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Supplementary tables: A novel click chemistry based peptide ELISA protocol: development and technical evaluation

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posted on 02.03.2022, 13:56 by Lisa Milchram, Regina Soldo, Valerie Regele, Silvia Schönthaler, Markus Degeorgi, Sophie-Kathrin Baumgartner, Elena Kopp, Andreas Weinhäusel

Supplementary Table 1: ratio of absorbance values for blocked versus unblocked wells for 2 peptides (EBV1, EBV2) in four dilutions steps (30, 60, 150 and 300 pmol per well) and different blocking agents (none, BSA and MP) for detection antibody (detAb) solution.

Supplementary Table 2: t-statistics of the comparison of absorbance values for N- & C-terminal orientation of the peptides immobilized as BSA-conjugates in HighBind plates and Maleimide coated plates

Funding

This work received funding from FFG Research Studios Austria (5th call, grant agreement no. 859182, project PepPipe) and H2020 Research and Innovation (grant agreement no. 633937, project ULTRAPLACAD). The funding organization was not involved in the design of the experiments. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.

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