Supplementary File.doc (10.21 MB)
Supplementary material for TEG-seq: an ion torrent-adapted NGS workflow for in cellulo mapping of CRISPR specificity
dataset
posted on 2019-11-27, 14:06 authored by Pei-Zhong Tang, Bo DingBo Ding, Lansha Peng, Vadim Mozhayskiy, Jason Potter, Jonathan D. Chesnut|
Supplementary Figure 1: Ion-GUIDE-seq workflow
Supplementary Figure 2: PCR amplification using different primer pairs
Supplementary Table 1: gRNA sequence and In silico design score from MIT design tool
Supplementary Table 2: TEG-seq and targeted amplicon-seq data
Supplementary Table 3: Data from PGM and Proton for HEK4
Supplementary Table 4: 22 gRNAs designed to target eight genetic disorder relevant SNPs
Supplementary Table 5: Oligo sequences of different primers and adaptors
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