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Supplementary material for TEG-seq: an ion torrent-adapted NGS workflow for in cellulo mapping of CRISPR specificity

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posted on 27.11.2019 by Pei-Zhong Tang, Bo Ding, Lansha Peng, Vadim Mozhayskiy, Jason Potter, Jonathan D. Chesnut

Supplementary Figure 1: Ion-GUIDE-seq workflow

Supplementary Figure 2: PCR amplification using different primer pairs

Supplementary Table 1: gRNA sequence and In silico design score from MIT design tool

Supplementary Table 2: TEG-seq and targeted amplicon-seq data

Supplementary Table 3: Data from PGM and Proton for HEK4

Supplementary Table 4: 22 gRNAs designed to target eight genetic disorder relevant SNPs

Supplementary Table 5: Oligo sequences of different primers and adaptors

Protocol for TEG-seq: An Ion Torrent-adapted NGS workflow for in cellulo mapping of CRISPR specificity

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