Supplementary material 1: Agarose gel electrophoresis to assess PCR product yield: comparison with spectrophotometry, fluorometry and qPCR
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Supplemental figure 1A and B: Agarose gel after PCR. A: Original image of agarose gel. B: Image of agarose gel with virtually subtracted background. Supplemental figure 2A and B: Agarose gel after purification of PCR products. A: Original image of agarose gel. B: Image of agarose gel with virtually subtracted background. Supplementary material 1: Protocol for Agarose gel electrophoresis to assess PCR product yield – Comparison with spectrophotometry, fluorometry and qPCR. Supplementary table 1: PCR reaction composition Supplementary table 2: PCR reaction parameters Supplementary table 3: Primer sequences Supplementary table 4: Peak area acquired with ImageJ from agarose gel after PCR Supplementary table 5: Peak area acquired with ImageJ from agarose gel after PCR product purification Supplemental material 3 containing: Supplementary table 6: Results of spectrophotometry Supplementary table 7: Results of fluorometry Supplementary table 8: Results of qPCR |
