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Supplementary Tables: Effect of mismatch between types of viral nucleic acid and intended targets of extraction kits on PCR-based testing

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posted on 2022-06-28, 07:58 authored by Jin-Ho Kim, Hanjun Kim, Kyoung-Jin Yoon

Supplementary Table 1. The summary of the designed primer sets information for the detection of respective viruses

Supplementary Table 2. Composition of the reaction mixture (in µL) for conventional PCR assays performed on nucleic acid extracted from bovine herpesvirus type 1 (BHV-1), porcine epidemic diarrhea virus (PEDV), porcine circovirus type 2 (PCV-2), and porcine rotavirus type A (PoRVA)

Supplementary Table 3. PCR conditions for conventional PCR assays performed on nucleic acid extracted from bovine herpesvirus type 1 (BHV-1), porcine epidemic diarrhea virus (PEDV), porcine circovirus type 2 (PCV-2), and porcine rotavirus type A (PoRVA)

Supplementary Table 4. Composition of the reaction mixture (in µL) for real-time (r) PCR assays performed on nucleic acid extracted from bovine herpesvirus type 1 (BHV-1), porcine epidemic diarrhea virus (PEDV), porcine circovirus type 2 (PCV-2), and porcine rotavirus type A (PoRVA)

Supplementary Table 5. PCR conditions for real-time (r) PCR assays performed on nucleic acid extracted from bovine herpesvirus type 1 (BHV-1), porcine epidemic diarrhea virus (PEDV), porcine circovirus type 2 (PCV-2), and porcine rotavirus type A (PoRVA)

Supplementary Table 6. Summary of the amplification sensitivity results of real-time (r) PCR assays

Funding

The study was supported in part by ISU VDL’s internal fund for assay R&D. Dr. Jin-Ho Kim was supported by the National Research Foundation of Korea (NRF-2018K1A4A3A01064257). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.

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