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Supplementary Tables: A qPCR-based method for rapid quantification of six intestinal homeostasis relevant bacterial genera in feces

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posted on 2021-08-03, 10:35 authored by Xinyi Gu, Tao Yu, Tingting Guo, Libei (Jiang Libei) KONGLibei (Jiang Libei) KONG

Supplementary Table 1. Bacterial species and strains used for six genus-specific primer pairs designa

a16S rRNA sequences of above species or strains covering Bacteroides, Lactobacillus, Pediococcus, Enterococcus, Streptococcus, Bifidobacterium, Prevotella, Clostridium, Eubacterium, Ruminococcus, Leuconostoc, E. coli and Klebsiella pneumoniae were retrieved from the NCBI GenBank database, and aligned to determine the regions designed for primers. The bacteria in the same box were closely related.

Supplementary Table 2. Level of similarity for 16S rRNA gene of 13 bacterial genera mentioned in Table S1.

16S rRNA gene sequence of 13 different bacterial genera used in Table S1 were aligned with each other, respectively. The similarity levels were detected by DNAMAN8.0.

Supplementary Table 3. Comparison between available primers and novel primers designed for six bacterial genera in this study.


This study was supported by a grant from the National Key Research and Development Program of China (2017YFD0400300, Beijing) and (2019YFA09006700).