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posted on 30.07.2021, 08:43 by Zewu Zhu, Xiaoqiong Zhang, Yingcheng Jiang, Shuhao Ruan, Fang Huang, Huimin Zeng, Minghui Liu, Weiping Xia, Feng Zeng, Jinbo Chen, Yu Cui, Hequn Chen
Supplementary Table 1. The characteristics of included 13 patients undergoing nephrectomy due to renal cancers.
Supplementary Table 2. Primer sequences for plasmid construction to overexpress NEAT1 and EGR1
Supplementary Table 3. ShRNA sequences designed for NEAT1 and BMP2
Supplementary Table 4. Sequences of Mimic and inhibitor for miRNAs
Supplementary Table 5. Primer sequences for qRT-PCR
Supplementary Table 6. The details of primary antibodies used in Western Blotting (WB)
Supplementary Table 7. Primer sequences for CHIP-qPCR
Supplementary Table 8. The transcription factors (TFs) binding to the promoter of BMP2 predicted by FIMO software, JASPAR and HOCOMOCO datasets, and RNA-Protein Interaction Prediction (RPISeq; http://pridb.gdcb.iastate.edu/RPISeq/#) was used to predict whether these TFs bind to NEAT1.
Supplementary Table 9. The predicted sites of EGR1 binding to the promoter of BMP2.

Funding

This work was supported by the National Natural Science Foundation of China (81770705 to Hequn Chen; 82000761 to Yu Cui) and Natural Science Foundation of Hunan Province (2017JJ3482 to Xiaoqiong Zhang).

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