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Supplementary Material. The histone modification H3K4me3 is altered at the ANK1 locus in Alzheimer’s disease brain.

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posted on 29.03.2021, 13:15 by Adam R Smith, Rebecca G Smith, Ruby Macdonald, Sarah J Marzi, Joe Burrage, Claire Troakes, Safa Al-Sarraj, Jonathan Mill, Katie Lunnon

Supplementary Material. The histone modification H3K4me3 is altered at the ANK1 locus in Alzheimer’s disease brain.

Background

Several epigenome-wide association studies of DNA methylation have highlighted altered DNA methylation in the ANK1 gene in Alzheimer’s disease brain samples. However, no study has specifically examined ANK1 histone modifications in the disease.

Methods

We use chromatin immunoprecipitation-qPCR to quantify tri-methylation at histone 3 lysine 4 (H3K4me3) and 27 (H3K27me3) in the ANK1 gene in entorhinal cortex from donors with high (N= 59) or low (N=29) Alzheimer’s pathology.

Discussion

We demonstrate decreased levels of H3K4me3, a marker of active gene transcription, with no change in H3K27me3, a marker of inactive genes. H3K4me3 is negatively correlated with DNA methylation in specific regions of the ANK1 gene.

Conclusions

Our study suggests that the ANK1 gene shows altered epigenetic marks indicative of reduced gene activation in Alzheimer’s disease.


Funding

AS-PG-14-038

NIRG-14-320878

ARUK-PPG2017B-021

MR/N027973/1

AG036039

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