Supplementary Files – Cloning vectors for gene delivery, integration, and expression in Campylobacter jejuni

dataset

posted on 2022-04-13, 10:19 authored by Figshare Future Science GroupFigshare Future Science Group, Gaylen A. Uhlich, Lori Bagi, Nereus W. Gunther IV

Supplemental file 1: pBlueKan+cysMPro- pBluescriptII KS+ with cloned aph(3), cysM promoter, and unique 3’ SmaI and XhoI cloning sites; KnR; ApR

Supplemental file 2: pCJR01- pBC SK::rRNA with unique NotI cloning site; CmR

Supplemental file 3: pBlueKan+cysMPro-flaA- pBlueKan+cysMPro with Gibson-cloned flaA; KnR; ApR

Supplemental file 4: pBlueKan+cysMPro-flaAB- pBlueKan+cysMPro with Gibson-cloned flaAB; KnR; ApR

Supplemental file 5: pCJR01comp-flaA- pCJR01 with cloned aph(3), PcysM, and flaA; KnR; CmR

Supplemental file 6: pCJR01comp-flaAB- pCJR01 with cloned aph(3), PcysM, and flaAB; KnR; CmR

Funding

USDA - Agricultural Research Service, National Program 108 Food Safety [Project # 8072-42000-076].

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Cloning vectors for gene delivery, integration, and expression in Campylobacter jejuni

BioTechniques

Keywords

Campylobacter restored function gene expression plasmids fimbriae

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CC BY-NC-ND 4.0