Supplementary File 3. Early life stress induces genome-wide sex-dependent miRNA expression and correlation across limbic brain areas in rats
Aims: The aim of this study was to assess regional- and sex-dependent changes in miRNA expression resulting from early-life stress (ELS).
Materials and Methods: Small RNA sequencing was used to determine sex-dependent changes in microRNAs after maternal separation (MS), a rodent model of ELS, across prefrontal cortex (PFC), amygdala, and hippocampus.
Results: Results showed that MS induced anhedonia and altered microRNA expression in a sex-dependent manner, particularly in PFC and hippocampus. Gene ontology revealed that these microRNAs target genes with brain-specific biological functions.