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Supplementary File 1. Early life stress induces genome-wide sex-dependent miRNA expression and correlation across limbic brain areas in rats

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posted on 20.05.2021, 09:35 by Lauren Allen McKibben, Yogesh Dwivedi

Aims: The aim of this study was to assess regional- and sex-dependent changes in miRNA expression resulting from early-life stress (ELS).

Materials and Methods: Small RNA sequencing was used to determine sex-dependent changes in microRNAs after maternal separation (MS), a rodent model of ELS, across prefrontal cortex (PFC), amygdala, and hippocampus.

Results: Results showed that MS induced anhedonia and altered microRNA expression in a sex-dependent manner, particularly in PFC and hippocampus. Gene ontology revealed that these microRNAs target genes with brain-specific biological functions.

Conclusions: Using a network approach to explore microRNA signaling across the brain after ELS, regional differences were highlighted as key to studying the brain’s stress response, which indicates that sex is critical for understanding microRNA-mediated ELS-induced behavior.

Funding

U.S. Department of Health and Human Services, National Institutes of Health, National Institute of Mental Health (MH082802; MH101890; MH100616; MH107183; MH112014)

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