Quercetin nanocrystals prepared by a novel technique improve dissolution rate and antifibrotic activity of quercetin - Supplementary materials

Aim: To develop quercetin nanocrystals by a simple approach and to evaluate their in vivo antifibrotic

efficacy. Materials & methods: Nanosuspensions were fabricated by a thin-film hydration technique and

ultrasonication. The influence of process variables on the average diameter of quercetin nanoparticles was

investigated. Moreover, in vivo efficacy was investigated in an established murine CCl4-induced fibrosis

model. Results: Nanocrystals showed a particle size of <400 nm. The optimized formulations showed an

increase in dissolution rate and solubility. Quercetin nanocrystals markedly prevented fibrotic changes in

the liver, as evidenced by mitigated histopathological changes and diminished aminotransferase levels and

collagen accumulation. Conclusion: The findings reflect the promising potential of quercetin nanocrystals

for liver fibrosis prevention.