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Proteomic identification of metabolic changes in Paracoccidioides brasiliensis induced by a nitroheteroarylchalcone - Supplementary files

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posted on 2023-07-10, 09:05 authored by Marcos ABC Junior, Lıvia C Silva, Olivia B Rocha, Amanda A Oliveira, Igor G Portis, Antonio Alonso, Lais Alonso, Kleber SF Silva, Marcelo N Gomes, Carolina H Andrade, Celia MA Soares, Maristela Pereira

Aim: To access the metabolic changes caused by a chalcone derivative (LabMol-75) through a proteomic

approach. Methods: Proteomic analysis was performed after 9 h of Paracoccidioides brasiliensis yeast

(Pb18) cell incubation with the LabMol-75 at MIC. The proteomic findings were validated through in vitro

and in silico assays. Results: Exposure to the compound led to the downregulation of proteins associated

with glycolysis and gluconeogenesis, β-oxidation, the citrate cycle and the electron transport chain.

Conclusion: LabMol-75 caused an energetic imbalance in the fungus metabolism and deep oxidative stress.

Additionally, the in silico molecular docking approach pointed to this molecule as a putative competitive

inhibitor of DHPS.


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