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Preparation of a shared chip for cell capturing and imaging with nanoparticle scaffold, nanogap and circulating tumor cell detection supplementary information

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posted on 2023-05-18, 10:36 authored by Yin-Qiu Lu, Qi Zeng, Huan-Huan He, Jing-Shuo Zhang, Hong-Ping Xie

Aim: To achieve accurate detection of circulating tumor cells (CTCs) in a large-volume sample. Materials &

methods: Silica nanoparticles were crosslinked layer-by-layer on glass slides as the substrate of a chip using

polyacrylic acid. Polyacrylic acid was immobilized as a spacer and capture ligands were immobilized on the

spacer. Results: The chip can be integrally applied to capture, post-treatment and imaging detection for

CTCs. The detected cell numbers were 33 and 40 for 9 cell/ml samples and clinical blood samples (7.5 ml),

respectively. The detection ratio of positive samples was 100%. Conclusion: The significantly increased

detected number for CTCs indicates that this methodology may avoid or greatly reduce the false-negative

ratio of positive clinical samples.

Funding

Priority Academic Program Development of the Jiangsu Higher Education Institutes, (Grant / Award Number: )

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