Mass spectrometric analysis of bioactive conditioned media of bacteria isolated from reptilian gut: supplementary table

Aim: To determine whether selected gut bacteria of crocodile exhibit antibacterial properties. Materials &

methods: Two bacteria isolated from Crocodylus porosus gut were used, namely: Pseudomonas aeruginosa

and Aeromonas dhakensis. Conditioned media were tested against pathogenic bacteria and metabolites

were analyzed using liquid chromatography-mass spectrometry. Results & conclusion: Antibacterial

assays revealed that conditioned media showed potent effects against pathogenic Gram-positive

and Gram-negative bacteria. LC–MS revealed identity of 210 metabolites. The abundant metabolites

were, N-Acetyl-L-tyrosine, Acetaminophen, Trans-Ferulic acid, N, N-Dimethylformamide, Pyrocatechol,

Cyclohexanone, Diphenhydramine, Melatonin, Gamma–terpinene, Cysteamine, 3-phenoxypropionic acid,

Indole-3-carbinol, Benzaldehyde, Benzocaine, 2-Aminobenzoic acid, 3-Methylindole. These findings

suggest that crocodile gut bacteria are potential source of novel bioactive molecules that can be utilized

as pre/post/antibiotics for the benefit of human health.