LMP1 encoded by Epstein–Barr virus mayactivate AHR through the ERK pathway innasopharyngeal carcinoma cells: supplementary files

Aim: To investigate the role of AHR in nasopharyngeal carcinoma (NPC) and explore the relationship

between Epstein–Barr virus (EBV) infection and the AHR pathway. Methods: The effect of LMP1 on the

expression of AHR was analyzed using real-time PCR and western blot. Proliferation and migration of cells

were assessed using CCK8 and Transwell analysis. Results: EBV infection downregulated the expression

of AHR in NPC cells, possibly through activation of the ERK pathway by LMP1 thereby accelerating

AHR proteasomal degradation following translocation to the nucleus. Cell proliferation, migration and

autophagy are promoted by activating the AHR pathway. Conclusion: In NPC cells, LMP1 increases the

phosphorylation of ERK, which may activate the AHR pathway.