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In silico analysis of ACE2 from different animal species provides new insights into SARS-CoV-2 species spillover: supplementary materials

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posted on 2023-04-11, 15:26 authored by Felipe Pantoja Mesquita, Pedro Filho Noronha Souza, Dyane Rocha Aragao, Expedito Maia Diogenes, Emerson Lucena da Silva, Jackson Lima Amaral, Valder Nogueira Freire, Debora de Souza Collares Maia Castelo-Branco, Raquel Carvalho Montenegro

Aim: This study aimed to analyze the phylogenetic relationships between the ACE2 of humans and

other animals and investigate the potential interaction between SARS-CoV-2 RBD and ACE2 of different

species. Materials & methods: The phylogenetic construction and molecular interactions were assessed

using computational models. Results & conclusion: Despite the evolutionary distance, 11 species had a

perfect fit for the interaction between their ACE2 and SARS-CoV-2 RBD (Chinchilla lanigera, Neovison

vison, Rhinolophus sinicus, Emballonura alecto, Saccopteryx bilineata, Numida meleagris). Among them,

the avian N. meleagris was reported for the first time in this study as a probable SARS-CoV-2 host due to

the strong molecular interactions. Therefore, predicting potential hosts for SARS-CoV-2 for understanding

the epidemiological cycle and proposal of surveillance strategies


The Office to Coordinate Improvement of Higher Education Personnel (CAPES) provided a postdoctoral grant to FP Mesquita. This work was also supported by grants from the following Brazilian agencies: National Council for Scientific and Technological Development, for a research productivity grant to RC Montenegro; CAPES for the grant to RC Montenegro (no. 88881.505364/2020- 01-Announcement 9/2020); Cearense Foundation to Support Scientific and Technological Development for the grant to RC Montenegro (no. 03195011/2020). RC Montenegro and DSCM Maia Castelo-Branco. The School of Medicine of Federal University of Ceara┬┤ also supported this research through a COVID-19 research grant.


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