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Hyperthermia and doxorubicin release by Fol-LSMO nanoparticles induce apoptosis and autophagy in breast cancer cells - Supplementary dataset

dataset
posted on 2023-01-16, 10:58 authored by Neha Kulkarni-Dwivedi, Pratikshkumar R Patel, Bhupendra V Shravage, Rinku D Umrani, Kishore M Paknikar, Sachin H Jadhav

Background: Studies on the anticancer effects of lanthanum strontium manganese oxide (LSMO)

nanoparticles (NPs)-mediated hyperthermia at cellular and molecular levels are scarce. Materials &

methods: LSMO NPs conjugatedwith folic acid (Fol-LSMO NPs) were synthesized, followed by doxorubicinloading

(DoxFol-LSMO NPs), and their effects on breast cancer cells were investigated. Results:

Hyperthermia (45◦C) and combination treatments exhibited the highest (∼95%) anticancer activity with

increased oxidative stress. The involvement of intrinsic mitochondria-mediated apoptotic pathway and

induction of autophagy was noted. Cellular and molecular evidence confirmed the crosstalk between

apoptosis and autophagy, involving Beclin1, Bcl2 and Caspase-3 genes with free reactive oxygen species

presence. Conclusion: The study confirmed hyperthermia and doxorubicin release by Fol-LSMO NPs induces

apoptosis and autophagy in breast cancer cells.

Funding

MACS-ARI, Pune, (Grant / Award Number: 'NBS07')

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