3 files

Human periodontal ligament stem cells with distinct osteogenic potentials induce bone formation in rat calvaria defects. Supplementary data

posted on 16.03.2022, 08:22 by Marcio Mateus BELOTI, Denise Carleto Andia, Karina Gonzales Silverio RUIZ, Rahyza Inácio Freire de ASSIS, Ana Carolina Loyola Barbosa, Gabriela Guaraldo Campos Totoli, Denise Weffort, Gileade Pereira FREITAS, Helena Bacha LOPES, Leticia Faustino Adolpho

Figure S1. Unlabeled periodontal ligament stem cells with low osteogenic potential (LP-PDLSCs) injected in calvarial bone defects 4 weeks (4 wks) post-treatment. The low intensity of red areas indicates the absence of PDLSC autofluorescence and confirms the presence of labeled PDLSCs in the calvarial bone defects shown in Figure 2. Scale bar = 50 µm.

Table S1. Primer sequences for real-time PCR

Table S2. Detection of human DNA in organs (average Ct ± standard deviation) of rats that received a local injection of periodontal ligament stem cells (PDLSCs) with high (HP-PDLS) and low (LP-PDLSCs) osteogenic potential and of phosphate buffered saline (PBS) in calvarial defects, 4 weeks post-injection


2019/10076-0, 2017/12622-7