Fungicidal and antibiofilm activities of gold nanoparticles on Candida tropicalis supplementary dataset

Aim: To investigate the antifungal activity of two different functionalized gold nanoparticles (AuNP),

those stabilized with cetyltrimethylammonium bromide and those conjugated with cysteine, and their

effects on the architecture of Candida tropicalis biofilms. Materials & methods: Biofilms were studied by

crystal violet binding assay and scanning electron microscopy. We investigated the effects of AuNPs on

reactive oxygen species, reactive nitrogen intermediates, and enzymatic and nonenzymatic antioxidant

defenses. Results/Conclusion: The fungicidal activity and cellular stress of both AuNPs affected biofilm

growth through accumulation of reactive oxygen species and reactive nitrogen intermediates. However,

cetyltrimethylammonium bromide-stabilized AuNPs revealed a higher redox imbalance.We correlated, for

the first time, AuNP effects with the redox imbalance and alterations in the architecture of C. tropicalis

biofilms.