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Dual-targeting exosomes for improved drug delivery in breast cancer- supplementary materials

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posted on 2023-05-17, 11:45 authored by Nam HB Tran, Diem DN Nguyen, Ngoc Mai Nguyen, Chau Tran, Ngoc Thanh Nguyen Thi, Duyen TK Ho, Hoai-Nghia Nguyen, Lan N Tu

Aims: The authors investigated whether displaying more than one homing peptide enhanced the tumor-targeting

efficiency of exosomes. Materials & methods: Exosomes from human embryonic kidney cells (HEK293F) were engineered to display either mono- or dual-tumor-penetrating peptides, iRGD and tLyp1. Exosomes were purified via tangential flow filtration followed by ultracentrifugation. Results: When loaded with doxorubicin (Dox), the dual iRGD-tLyp1 exosomes strongly enhanced Dox uptake in both MCF-7 and MDA-MB-231 breast cancer cell lines, superior to single iRGD or tLyp1 exosomes. The dual iRGDtLyp1 exosomal Dox was also the most potent, with IC50/GI50 values being 3.7–17.0-times lower than those of free Dox and other exosomal Dox. Conclusion: Selecting appropriate combinatorial homing peptides could be an approach for future precision nanomedicine.

Funding

NexCalibur Therapeutics, (Grant / Award Number: )

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