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Development of a sensitive HPLC method with fluorescence detection for determination of motilin in human plasma: supplementary materials

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posted on 2023-04-04, 19:58 authored by Sakine Atila Karaca, Tuncer Temel, Duygu Yeniceli Ugur

Background: Motilin is a peptide-structured gastrointestinal system hormone. In this study, a sensitive

HPLC-fluorescence detection method was developed and validated for the quantification of motilin in

human plasma. Materials & methods: Optimization processes were carried out with the experimental

design methodology. Analyses were performed on a C8 column (4.6 × 150 mm, 3.5 μm particles) using

water and acetonitrile containing trifluoroacetic acid as the mobile phase. Results & conclusion: The

method was linear from 2 to 200 ng/ml of motilin. The assay variability was less than 5%. The limit of

quantification was found to be 1.84 ng/ml. The applicability of the developed method was successfully

demonstrated by quantifying the levels of motilin in human plasma samples.

Funding

The authors appreciate Research Council of Anadolu University for the support of the project (project no. 1807S252).

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