Development and validation of methods that enable high-quality droplet digital PCR and hematological profiling data from microvolume blood samples: Supplementary tables

Aim: Mouse models have been crucial to preclinical studies in the increasingly relevant fields of cell and

gene therapy. However, only small quantities of mouse blood can be collected without producing adverse

physiological effects that compromise data integrity. Results: To address this limitation, two combined

methods were developed to create detailed droplet digital PCR (ddPCR) and hematological profiles using

only ∼20 μl of mouse blood. The validation of these methods, which can serve as a foundation for a

standardized regulatory pipeline for ddPCR, is discussed. Even when using small amounts of input, this

ddPCR protocol is accurate, precise, selective, specific, stable and robust. Conclusion: These techniques

enable more frequent sample collection for higher-resolution pharmacokinetic data that meets or exceeds

quality standards.