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Determination of drug-to-antibody ratio of antibody–drug conjugate in biological samples using microflow-liquid chromatography/high-resolution mass spectrometry: Supplementary materials

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posted on 2023-02-24, 14:15 authored by Kazuko Inoue, Toshiki Mochizuki, Nana Kasamori, Takafumi Komori

Background: Antibody–drug conjugates (ADCs) are a promising modality for cancer treatment; however,

considering their complicated nature, analytical complexity in understanding their pharmacokinetics and

pharmacodynamics in the body presents a significant challenge. Results: Vorsetuzumabmaleimidocaproyl

valine-citrulline p-aminobenzyloxycarbonyl monomethyl auristatin E was used to develop pretreatment

and analytical workflows suitable for ADCs. Monomethyl auristatin E release and drug-to-antibody ratio

retention were consistent in mouse plasma but inconsistent in monkey and human plasma. Further,

metabolites were species-specific. Microflow-liquid chromatography/high-resolution mass spectrometry

(LC–HRMS) resulted in a 4–7-fold improvement in detection sensitivity compared with conventional flow

LC–HRMS. Conclusion: Microflow-LC–HRMS can be a useful tool in understanding the complex properties

of ADCs in the body from a drug metabolism and pharmacokinetics point of view.