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Detection of colonization capacity of probiotic Bifidobacterium breve CCFM1025in the human gut - Supplementary Table

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posted on 2023-06-15, 15:02 authored by Xin Qian, Peijun Tian, Guopeng Lin, Xinglong Xu, Gang Wang, Hao Zhang, Wei Chen

Aim: To detect the gut colonization capacity of Bifidobacterium breve CCFM1025 with clinical

antidepressant-like effects. Materials & methods: A unique gene sequence of B. breve CCFM1025 was discovered based on the genome analysis of 104 B. breve strains and a strain-specific primer (1025T5) was designed. In vitro and in vivo samples were used to validate the specificity and quantitative capability of this primer in the PCR system. Results: Quantitative PCR using strain-specific primers enabled absolute quantification of CCFM1025 in fecal samples within 104–1010 cells/g (R2 >0.99). CCFM1025 remained highly detectable in volunteer feces 14 days after cessation of administration, demonstrating its favorable colonization characteristics. Conclusion: CCFM1025 can colonize the healthy human gut.


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