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Comparison of Bruker Biotyper (R) and Vitek (R) - MS matrix-assisted laserdesorption/ionization–time-of-flight massspectrometry platforms for the identificationof filamentous fungi - Supplemental Table 2

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posted on 2023-06-15, 14:54 authored by Julia D Hankins, Megan H Amerson-Brown, Cameron A Brown, Ashleigh N Riegler, Kenneth L Muldrew, James J Dunn

Aims: To evaluate the performance of twomatrix-assisted laser desorption/ionization–time-of-flight mass spectrometry platforms to identify molds isolated from clinical specimens. Methods: Fifty mold isolates were analyzed on Bruker Biotyper (R) and Vitek (R) MS platforms. Two Bruker Biotyper extraction protocols were assessed alongside the US FDA-approved extraction protocol for Vitek MS. Results: The Bruker Biotyper modified NIH-developed extraction protocol correctly identified more isolates than Bruker’s protocol (56 vs 33%). For species in the manufacturers’ databases, Vitek MS correctly identified 85% of isolates, with 8% misidentifications. The Bruker Biotyper identified 64%, with no misidentifications. For

isolates not in the databases, the Bruker Biotyper did not misidentify any, and VitekMSmisidentified 36%. Conclusion: Both the VitekMS and Bruker Biotyper accurately identified the fungal isolates, however Vitek MS was more likely to misidentify isolates than the Bruker Biotyper.