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Application of a new volumetric microsampling device for quantitative bioanalysis of immunosuppression: Supplementary material

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posted on 2022-10-08, 08:57 authored by Abbie D Leino, John Takyi-Williams, Bo Wen, Duxin Sun, Manjunath P Pai

Background: Volumetric absorptive microsampling may reduce the blood collection burden associated

with therapeutic drug monitoring of immunosuppression to prevent organ transplant rejection. This

work describes the development of a laboratory and analytical technique for quantifying tacrolimus and

mycophenolic acid (MPA) from the Tasso-M20™ in human whole blood using bead-based impact-assisted

extraction. Results: The sampled blood volume was accurate with estimated volumes within <2% of the

expected 20 μl. Recovery using impact-assisted extraction was 73–87% for MPA and 100% for tacrolimus

and was hematocrit-independent for both analytes. The LC-MS/MS assay is precise and accurate within

the acceptance criteria of 15%. Conclusion: The sampling and extraction procedures allowed for accurate

quantification of tacrolimus and MPA. Exploration of abuse scenarios identified important education

points for patients conducting home-based sample collections in the future.

Funding

Administrative Supplements for the NCI P30 Cancer Center Support Grants for Multi-Channel Communication Campaigns for Improvements in Cancer Education and Outcomes (MICEO) in Underserved Populations

National Cancer Institute

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