Application of a new volumetric microsampling device for quantitative bioanalysis of immunosuppression: Supplementary material
Background: Volumetric absorptive microsampling may reduce the blood collection burden associated
with therapeutic drug monitoring of immunosuppression to prevent organ transplant rejection. This
work describes the development of a laboratory and analytical technique for quantifying tacrolimus and
mycophenolic acid (MPA) from the Tasso-M20™ in human whole blood using bead-based impact-assisted
extraction. Results: The sampled blood volume was accurate with estimated volumes within <2% of the
expected 20 μl. Recovery using impact-assisted extraction was 73–87% for MPA and 100% for tacrolimus
and was hematocrit-independent for both analytes. The LC-MS/MS assay is precise and accurate within
the acceptance criteria of 15%. Conclusion: The sampling and extraction procedures allowed for accurate
quantification of tacrolimus and MPA. Exploration of abuse scenarios identified important education
points for patients conducting home-based sample collections in the future.
Cancer Center Support Grant 2018-2023
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