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Antifungal activity of cisatracurium against fluconazole-resistant Candida isolates and its antibiofilm effects - Supplementary material

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posted on 2023-08-04, 14:59 authored by Francisca BA do Nascimento, L´ıvia GA Valente Sa, Joa˜o B de Andrade Neto, Vito´ ria PF Cabral, Daniel S Rodrigues, Amanda D Barbosa, Lara EA Moreira, Leilson C Oliveira, Anderson Silva, Iri SP Lima, Jacilene Silva, Emmanuel S Marinho, H´ elcio S Santos, Bruno C Cavalcanti, Manoel O Morais, He´ lio VN Ju´ nior, Cec´ılia R Silva

Aim: To evaluate the antifungal activity of cisatracurium against Candida spp. resistant to fluconazole strains in planktonic and biofilm forms, in addition to determining its mechanism of action. Materials & methods: Antifungal activity and pharmacological interactions were determined using broth microdilution methods and the mechanism of action was evaluated by flow cytometry and molecular docking. Results: Cisatracurium presented antifungal activity against Candida spp. planktonic cells due to alterations of mitochondrial transmembrane potential leading to cellular apoptosis in addition to interacting with important targets related to cellular respiration, membrane and cell wall evidenced by molecular docking. Furthermore, the drug both prevented biofilm formation and impaired mature biofilms. Conclusion: Cisatracurium exhibits potential antifungal activity against Candida spp.