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A validated method for the determination of hematocrit in dried blood spots using image analysis: Supplementary data

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posted on 2023-03-24, 15:13 authored by Miguel Gambell Barroso, Louise Gustafsson, Victoria Barcla, Camilla Linder

Aim: To develop a nondestructive method for the estimation of hematocrit (HCT) in dried blood spots

(DBSs). Materials & methods: Standards and controls were created (HCT range: 0.20–0.50 l/l) and DBS

scanned using a flatbed scanner. Gray values and pixel areas were analyzed with open-source software to

estimate HCT and volume, respectively. HCT obtained in whole blood using hematological analyzer was

compared with DBS scannermethod (n = 50). Results: Between-run precision was 4.7–10.2% and betweenrun

accuracy was 89.6–102.1%. In the hematological instrument comparison, 96% of the patient sample

results were within ±15%. Conclusion: The nondestructive method can be used to exclude patient DBS

samples with extreme HCT levels from further analysis and avoid bias on measured concentration.