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A systematic investigation of key factors of nucleic acid precipitation towards optimized DNA/RNA isolation

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posted on 18.02.2020 by Yalin Li, Suxiang Chen, Nan Liu, Lixia Ma, Tao Wang, Rakesh N. Veedu, Tao Li, Fengqiu Zhang, Huiyue Zhou, Xiang Cheng, Xumiao Jing

Supplementary Figure 1 - Key influence factors and their corresponding conditions of nucleic acid precipitation;

Supplementary Table 1 - The average recovery rate and its corresponding standard deviation of each type of nucleic acid by adding alcohol and salt independently or both;

Supplementary Table 2 - The average recovery rate and its corresponding standard deviation and significance level of each type of nucleic acid under each incubation condition;

Supplementary Table 3 - The average recovery rate and its corresponding standard deviation and significance level of each type of nucleic acid under each centrifugation force;

Supplementary Table 4 - The average recovery rate and its corresponding standard deviation and significance level of each type of nucleic acid under each centrifugation time;

Supplementary Table 5 - The average recovery rate and its corresponding standard deviation and significance level of each type of nucleic acid under each volume ratio of ethanol or isopropanol to nucleic acid solution;

Supplementary Table 6 - The average recovery rate and its corresponding standard deviation and significance level of each type of nucleic acid under different conditions of cations;

Supplementary Table 7 - The average recovery rate and its corresponding standard deviation of each type of nucleic acid upon addition of yeast tRNA as co-precipitator (carrier);

Supplementary Table 8 - The average recovery rate and its corresponding standard deviation and significance level of each type of nucleic acid under different conditions of carriers;

Supplementary Table 9 - The measuring results of carries in aqueous solutions by NanoDrop 2000.

Funding

This work was supported by the Natural Science Foundation of China (11704343, 11605159 and 81773175), China Postdoctoral Science Foundation (2018M630839), College Key Research Project in Henan province (18A180032), Science and Technology Research Project in Henan province (162102210155). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.

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