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A sensitive, robust and high-throughput isotope dilution LC–MS/MS method for quantifying three folate forms in serum: Supplementary data

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posted on 2023-03-28, 18:47 authored by Yunfeng Cao, Jia Zeng, Ting Huang, Jianxing Chen

This study aimed to establish an isotope dilution LC–MS/MS method for the determination of folic acid,

5-formyltetrahydrofolate and 5-methyltetrahydrofolate in human serum. This method was then used to

quantify these three folate forms in the healthy adult population and supplement users. A stable 96-

well solid-phase extraction system was used to prepare serum samples. The highly sensitive method was

established using a Shimadzu LCMS-8060NX. The linearity was good in the range of 0.1–10 nmol/l for

folic acid and 5-formyltetrahydrofolate and 1.0–100 nmol/l for 5-methyltetrahydrofolate. The accuracy

and precision were good. The method was sensitive, robust and high-throughput and could be used for

the routine clinical monitoring of these three folate forms in the Chinese population.


This work was funded by National Natural Science Foundation of China, grant nos. 82274123 and 82274403. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.