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ARG2 single-nucleotide polymorphism rs3742879 affects plasma arginase 2 levels, nitric oxide formation and antihypertensive therapy response in preeclampsia: supplementary files

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posted on 2022-08-16, 09:22 authored by Marcelo R Luizon, Caroline C Pinto-Souza, Fernanda Coeli-Lacchini, Riccardo Lacchini, Ricardo C Cavalli, Valeria C Sandrim

Aim: This work examined whether ARG1 (rs2781659, rs2781667, rs2246012 and rs17599586) and ARG2

(rs3742879 and rs10483801) single-nucleotide polymorphisms (SNPs) are associated with antihypertensive

therapy responsiveness in preeclampsia (PE) and their effects on arginase isoforms and nitrite

concentrations in responsive and nonresponsive patients. Methods: SNP genotypes were determined by

TaqMan assays. Plasma arginase levels were measured by ELISA and nitrite concentrations were measured

using an ozone-based chemiluminescence assay. Results: The G allele for ARG2 rs3742879 (A>G) was less

frequent in nonresponsive compared with responsive patients (15.5% vs 24.7%) and the G carriers of the

nonresponsive subgroup had lower arginase 2 (9.2 ± 7.5 ng/ml vs 19.1 ± 17.3 ng/ml) and higher nitrite

concentrations (110.2 ± 52.8 nM vs 78.5 ± 37.9 nM) than carriers of the AA genotype (all p < 0.05).

Conclusion: ARG2 SNP rs3742879 is associated with diminished arginase 2 levels and increased nitric oxide

formation in nonresponsive PE patients.


This work was supported by the National Council for Scientific and Technological Development (CNPq/Brazil, grant no. 2014- 5/305587 and 308504/2021-6), the Sao Paulo Research Foundation (FAPESP/Brazil, grant no. 2019/07230-8) and Coordination for the Improvement of Higher-Level Personnel (CAPES/Brazil, grant number financial code 001).


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