Supplementary data

Table S1. Oligonucleotide sequences used in this study

Figure S1. Library yield depending on the number and position of phosphorothioate bonds in the

adapter oligonucleotides. The used adapters contained different numbers of phosphorothioate

bonds at different positions within the oligonucleotide. Oligonucleotide designations: BC, barcode number of the Ion Xpress index sequence; pt, adapter oligonucleotide containing given number of

phosphorothioate bonds; bc, phosphorothioate bonds located upstream of the barcode within the

key sequence. For further details and the oligonucleotide sequences, see Table 1 in the main

document. All libraries were prepared of the same batch of fragmented E. coli DNA in two independent experiments; vertical lines indicate the standard deviation calculated from three dilutions used in qPCR quantification. The horizontal line indicates the mean concentration of all libraries shown in the plot, the dashed lines indicate the mean ± 1 standard deviation

Figure S2.