Supplementary Figure S4. Simultaneous multiple protein expression from a single vector for Dictyostelium

2019-12-11T16:24:04Z (GMT) by Tomo Kondo Shigehiko Yumura
Supplementary Figure S4. Simultaneous multiple protein expression from a single vector for Dictyostelium. (A) Scheme of tandem GOI alignment using seamless cloning (In-fusion cloning). After linearization of the vector containing GOI1 with ApaI (whose recognition site is not present in 99.5% of the Dictyostelium genes), a PCR product containing GOI2 with a promoter (orange) and terminator (cyan, shaded) was inserted. (B) A γ-tubulin–GFP expressing cell. G418 was used for selection. (C) A γ-tubulin–GFP and mRuby3–histone H1 expressing cell. Only G418 was used for selection. The right side of each image superimposed to the phase-contrast image. Bars, 10 µm.