Supplementary Figure 3.tif (14.95 MB)

Supplementary Figure S3. Plasmid map of pDYU1NeoTED

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posted on 2019-12-11, 16:24 authored by Tomo Kondo, Shigehiko Yumura

Supplementary Figure S3. Plasmid map of pDYU1NeoTED. Ddp1 (truncated) indicates a 2033 bp HindIII fragment of the pBIG vector (GenBank accession number AF270470). The TED module (1214 bp) contains 287 bp of the sequence upstream of the CAP binding site of pUC19. NeoR/KanR was replaced with bsd to create blasticidin S-resistant cell lines.

Funding

Tomo Kondo was supported by the Japan Society for the Promotion of Science Research Fellowships for Young Scientists. This research was supported by the Japan Society for the Promotion of Science [KAKENHI Grant Number 16J08310 and 19K15809 to TK]. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.

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E. coli molecular cloning screening GFP Dictyostelium Magnetospirillum Microbial Genetics Genomics

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