Figure S3.png (150.34 kB)
Supplementary Figure S3. High-throughput phenotyping of cell-to-cell interactions in gel microdroplet pico-cultures
figure
posted on 2019-04-17, 08:55 authored by Juliette Ohan, Benjamin Pellea, Pulak Nath, J.-H. Huang, Blake Hovde, Momchilo Vuyisich, Armand E.K.Dichosa, Shawn R. StarkenburgPost-cultivation
targeted FACS enrichment of abGMDs. Control population of cultivated aGMDs (A)
and experimental cultivated population of abGMDs (D) using FSC (x-axis; linear
scale) versus SSC (y-axis; log scale). (E) Cultivated abGMDs exhibiting chlorophyll
autofluorescence higher than that of the cultivated control aGMDs shown in (B)
using a 650/50 nm filter (x-axis at logarithmic scale) versus 530/40 nm filter
(y-axis at logarithmic scale). (F) Gated selection of cultivated abGMDs
exhibiting higher FSC (to distinguish from free algae) and SSC (to distinguish
from aGMDs without microcolonies) values than that of the uncultivated
controls. (C) Application of the same gating strategy shown in (F) to a
cultivated aGMD control.
