Supplementary Figure S2. Quantification of magnetosome signals and plasmid stability in MSR-1
Supplementary
Figure S2. Quantification of magnetosome signals and plasmid stability in
MSR-1. (A) Example of image processing to detect signals from
magnetotactic bacteria. Briefly, cell perimeters were detected from a
phase-contrast image (a). The detected boundaries were overlaid on the
fluorescent image (b). Finally, the signals were detected only inside the
cell (c). (B) Quantitative analysis of cells cultured for 25 days in the
presence or absence of ampicillin. Cells were passaged every 2–3 days. Each
number (#1–4) represents a cell line. Fluorescence (+) indicates cells with
HaloTag ligand signals. n ≥ 146. (C) Image of an 1% agarose gel after
electrophoresis of purified plasmids. E. coli indicates the lane with
plasmids purified from E. coli cells. Each number (#1–4) represents a cell
line of magnetotactic bacteria, which are the same ones shown in (B). All
plasmids were linearized by a restriction enzyme reaction using MluI. The
arrow indicates the predicted length of DNA bands. The gel edge was trimmed
by image processing but no band enhancement was performed. |