Supplementary Figure S2. Quantification of magnetosome signals and plasmid stability in MSR-1

2019-12-11T16:24:04Z (GMT) by Tomo Kondo Shigehiko Yumura

Supplementary Figure S2. Quantification of magnetosome signals and plasmid stability in MSR-1. (A) Example of image processing to detect signals from magnetotactic bacteria. Briefly, cell perimeters were detected from a phase-contrast image (a). The detected boundaries were overlaid on the fluorescent image (b). Finally, the signals were detected only inside the cell (c). (B) Quantitative analysis of cells cultured for 25 days in the presence or absence of ampicillin. Cells were passaged every 2–3 days. Each number (#1–4) represents a cell line. Fluorescence (+) indicates cells with HaloTag ligand signals. n ≥ 146. (C) Image of an 1% agarose gel after electrophoresis of purified plasmids. E. coli indicates the lane with plasmids purified from E. coli cells. Each number (#1–4) represents a cell line of magnetotactic bacteria, which are the same ones shown in (B). All plasmids were linearized by a restriction enzyme reaction using MluI. The arrow indicates the predicted length of DNA bands. The gel edge was trimmed by image processing but no band enhancement was performed.