Supplementary Figure 2. Rapid Identification of Genome Edited Mesenchymal Stem Cell Colonies via Cas9

Supplementary Figure 2. Target inserting the IL-37 into AASV1 site. (A) Donor and gRNA structures. (B) PCR characterization of genome edited MSC colonies by amplifying the left arm. Arrow shows the predicted PCR product size. (C) PCR characterization of genome edited MSC colonies by amplifying the right arm. Arrow shows the predicted PCR product size. (D) mRNA level of IL-37 among the colony 8 (C8) without donor insertion, colony 20 (C20) with correct insertion, and the wild type of MSCs (NC). M: DNA ladder; arrows showed the DNA size with 750bp, 1.0kb or 2kb.