Supplementary Figure 1. Development of a motion-based cell-counting system for Trypanosoma parasite using a pattern recognition approach

T. cruzi epimastigote cell count estimation by conventional methods. (A)TC20automated cell counter(Bio-Rad, Hercules, California, USA)was used to count rosette-rich epimastigote, according to the manufacturer’s instruction. Actual cell count was determined by using hemocytometer after breaking apart cell aggregates by pipetting and vortexing. Dottedlinerepresentsthetheoreticallineofperfectcorrelationbetweenactual cell counts and machine-estimated cell counts. (B)Fiji-ImageJ software[1] was used to count the same sample as(A),using threshold adjustment and watershed segmentation functions.(C)Cell Profiler Worm Toolbox[2] was used to count the same sample as(A).The same still images used in (B)were analyzed.