Supplemental Table S1. Oligonucleotides used to construct and verify a quadruple mutant in M. smegmatis. Supplemental Table S2. Oligonucleotides used to construct and verify a triple in-frame deletion mutant in M. tuberculosis.

This work was supported by Centre National de la Recherche Scientifique; Université Paul Sabatier; Agence Nationale de la Recherche (ANR14-CE14-0024), Fondation pour la Recherche Médicale (DEQ2016 0334902); Y.-M.B. is supported by FRM. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. No writing assistance was utilized in the production of this manuscript.