Nanoliter-scale next-generation sequencing library mediated high-throughput 16s RRNA microbial community profiling: Supplementary Tables

Supplementary Table 1. Nanoliter-scale next-generation sequencing library mediated high-throughput 16s RRNA microbial community profiling.

Primer sequences. The inner primer pairs were used to amplify the V3/V4 region of the 16S rRNA gene. The outer primers with spacer sequences and SP sequences at the 5´region of primer were used to construct SNAP-TE libraries compatible for Illumina MiSeq/HiSeq platform.

Supplementary Table 2. Nanoliter-scale next-generation sequencing library mediated high-throughput 16s RRNA microbial community profiling.

Index sequences used for next-generation sequencing library construction.

Supplementary Table 3. Nanoliter-scale next-generation sequencing library mediated high-throughput 16s RRNA microbial community profiling.

A total of 14 flexible sample/assay combination modes that can set on multi-sample nanodispenser system. Replicates can be easily done using these settings.

Supplementary Table 4. Nanoliter-scale next-generation sequencing library mediated high-throughput 16s RRNA microbial community profiling.

All parameters tested for SNAP-TE experiments. Two replicates of 24 nanowells on a chip were used for each test of PCR system conditions. Data from individual next-generation sequencing libraries from each nanowell were pooled by samples for analyses.

Supplementary Table 5. Nanoliter-scale next-generation sequencing library mediated high-throughput 16s RRNA microbial community profiling.