Nanoliter-scale next-generation sequencing library mediated high-throughput 16s RRNA microbial community profiling: Supplementary Tables
Supplementary Table 1.
Nanoliter-scale
next-generation sequencing library mediated high-throughput 16s RRNA
microbial community profiling.
Primer sequences. The inner primer pairs were used to amplify the V3/V4 region of the 16S rRNA gene. The outer primers with spacer sequences and SP sequences at the 5´region of primer were used to construct SNAP-TE libraries compatible for Illumina MiSeq/HiSeq platform.
Supplementary Table 2. Nanoliter-scale next-generation sequencing library mediated high-throughput 16s RRNA microbial community profiling.
Index sequences used for next-generation sequencing library construction.
Supplementary Table 3. Nanoliter-scale next-generation sequencing library mediated high-throughput 16s RRNA microbial community profiling.
A total of 14 flexible sample/assay combination modes that can set on multi-sample nanodispenser system. Replicates can be easily done using these settings.
Supplementary Table 4. Nanoliter-scale next-generation sequencing library mediated high-throughput 16s RRNA microbial community profiling.
All parameters tested for SNAP-TE experiments. Two replicates of 24 nanowells on a chip were used for each test of PCR system conditions. Data from individual next-generation sequencing libraries from each nanowell were pooled by samples for analyses.
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